Avtor/Urednik     Zore, Anamarija; Petrovec, Miroslav; Prosenc, Katarina; Trilar, Tomi; Ružić-Sabljić, Eva; Avšič-Županc, Tatjana
Naslov     Infection of small mammals with Borrelia burgdorferi sensu lato in Slovenia as determined by polymerase chain reaction (PCR)
Tip     članek
Vir     Wien Klin Wochenschr
Vol. in št.     Letnik 111, št. 22-23
Leto izdaje     1999
Obseg     str. 997-9
Jezik     eng
Abstrakt     Thirty-four small mammals collected in the vicinity of Ljubljana were tested for the presence of Borrelia burgdorferi sensu lato by polymerase chain reaction (PCR) of urinary bladder tissues, using universal flagellin primers and species specific rRNA primers. Seventeen small mammals (50%) were found to be positive, and 7 small mammals were infected with two species of B. burgdorferi sensu lato simultaneously. The most commonly found species was B. afzelii (n =14), followed by B. burgdorferi sensu stricto (n = 7) and B. garinii (n = 3), as determined by species-specific primers. We conclude that PCR is a rapid and reliable method to detect infection with B. burgdorferi sensu lato in small mammals.
Deskriptorji     BORRELIA BURGDORFERI
LYME DISEASE
DISEASE RESERVOIRS
MAMMALS
BLADDER
PREVALENCE
FLAGELLIN
BASE SEQUENCE
RNA, RIBOSOMAL, 16S
SLOVENIA