Avtor/Urednik		Battelino, Tadej; Miralles, Francisco; Kržišnik, Ciril; Scharfmann, Raphael; Czernichow, Paul
Naslov		TGF-beta activates genes identified by differential mRNA display in pancreatic rudiments
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 439, št. 3 Suppl
Leto izdaje		2000
Obseg		str. R26-8
Jezik		eng
Abstrakt		The effect of TGF-beta on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-beta. Differentially expressed cDNAs were re-amplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain alpha-tropomyosin cDNA were identified from the group of augmented cDnas, andB-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embrionic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified.
Deskriptorji		PANCREAS TRANSFORMING GROWTH FACTOR BETA RNA, MESSENGER GLUCAGON BLOTTING, NORTHERN RATS, WISTAR POLYMERASE CHAIN REACTION