Avtor/Urednik		Zajc-Kreft, Katarina; Kreft, Samo; Komel, Rado; Grubič, Zoran
Naslov		Nonradioactive northern blotting for the determination of acetylcholinesterase mRNA. Comparison to the radioactive technique
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 439, št. 3 Suppl
Leto izdaje		2000
Obseg		str. R66-7
Jezik		eng
Abstrakt		A sensitive nonradioactive northern blotting for the detection of acetylcholinesterase mRNA in mammalian tissues is described and compared to its radioactive version. Best results were obtained if digoxigenin labeled RNA probe was used for hybridization and CDP-StarTM, a chemiluminescent alkaline phosphatase substrate, for detection. The described nonradioactive technique for acetylcholinesterase mRNA determination is as sensitive as the radioactive one, but requires no protection against radiation and is less time consuming. Because of higher stability of the labeled probe, nonradioactive technique is also more convenient from the standpoint of experimental planning.
Deskriptorji		ACETYLCHOLINESTERASE RNA, MESSENGER MUSCLE, SKELETAL DIGOXIGENIN RATS BLOTTING, NORTHERN RNA PROBES