Avtor/Urednik		Kopitar-Jerala, Nataša; Bestagno, Marco; Fan, Xiaouhi; Novak-Despot, Djurdja; Burrone, Oscar; Kos, Janko; Škrk, Janez; Gubenšek, Franc
Naslov		Molecular cloning and chimerisation of CDI 315B monoclonal antibody
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 439, št. 3 Suppl
Leto izdaje		2000
Obseg		str. R79-80
Jezik		eng
Abstrakt		A chimeric mouse-human antibody has been created that recognizes an antigen found on breast cancer cells and melanoma cells. Immunoglobulin constant domains of mouse monoclonal antibody CDI 315B Cgamma1 and Ckappa, were substituted by the human Cgamma1 and Ckappa. The CDI 315B variable heavy and light chain regions were PCR amplified from hybridoma RNA and sequenced. Mouse variable VH and VL regions were joint to human IgG1 and kappa constant regions and subcloned into pcDNA3 expression vectors. The Sp2/0 murine myeloma cells were transfected with expression vectors pcDNA3L and pcDNA3H and the reactiviry of chimeric antibodies was tested by indirect ELISA using B16F1 murine melanoma cells as well as MCF7 human breast cancer cells, as antigen.
Deskriptorji		BREAST NEOPLASMS MELANOMA ANTIBODIES, MONOCLONAL MICE POLYMERASE CHAIN REACTION TUMOR CELLS, CULTURED