Avtor/Urednik		Makovec, Tomaž; Breskvar, Katja
Naslov		Purification of cytochrome P450 from filamentous fungus Rhyzopus nigricans
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 439, št. 3 Suppl
Leto izdaje		2000
Obseg		str. R111-12
Jezik		eng
Abstrakt		It has been reported in our previous studies that steroid hydroxylation system of Rhizopus nigricans involves cytochrome P450 and NADPH-cytochrome P450 reductase in the electron transport system. Both enzymes are membrane bound and are located in the microsomal preparations of progesterone induced fungal mycelia. In order to identify and characterize the cytochrome P450 component of fungal monoxygenase system, microsomal proteins from induced mycelia were subjected to HIGH Q anion exchange and MONO P (FPLC) anion exchange chromatography. Four fractions containing cytochrome P450 have been resolved on MONO P column. They exhibit CO difference spectra and type II difference spectra with ketoconazole.
Deskriptorji		RHIZOPUS CYTOCHROME P-450 CHROMATOGRAPHY, ION EXCHANGE