Avtor/Urednik		Chowdhury-Haque, H; Popoff, MR; Zorec, Robert
Naslov		Actin cytoskeleton and exocytosis in rat melanotrophs
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 439, št. 3 Suppl
Leto izdaje		2000
Obseg		str. R148-9
Jezik		eng
Abstrakt		We monitored secretory activity of single rat melanotrophs by the patch-clamp membrane capacitance measurements (Cm). Secretory activity was stimulated by cytosol dialysis with a patch-pipette solution containing 1 microM (Ca2+)i. Actin cytoskeleton was disaggregated by pretreating cells with Clostridium spiroforme toxin, which specifically ADP-ribosylates cellular actin. The extent of cytoskeleton disaggregation was monitored by phalloidin immunostaining. The maximal rate of secretion increases two folds in toxin-treated cells in comparison to controls, whereas the extent of calcium-induced secretory response was similar to that obtained in the non-treated cells. The results show that the subcortical actin network attenuates the rate of secretory activity, which we interpret to reflect a barrier function of cytoskeleton for exocytosis.
Deskriptorji		CYTOSKELETON EXOCYTOSIS ACTINS CALCIUM BACTERIAL TOXINS RATS, WISTAR CLOSTRIDIUM PITUITARY GLAND PATCH-CLAMP TECHNIQUES