Avtor/Urednik		Novaković, Srdjan; Ihan, Alojz; Jezeršek, Barbara
Naslov		Effectiveness of a simply designed tumor vaccine in prevention of malignant melanoma development
Tip		članek
Vir		Jpn J Cancer Res
Vol. in št.		Letnik 90
Leto izdaje		1999
Obseg		str. 1130-8
Jezik		eng
Abstrakt		We investigated the efficacy of a simple syngeneic tumor vaccine to induce specific antitumor immunity on female C57B1/6 mice. Tumor vaccine was prepared by mixing irradiated B-16 melanoma tumor cell with the pleiotropic biological response modifier-maleic anhydride divinyl ether (MVE-2). Experimental animals were pretreated with the vaccine in order to prevent the development of intraperitoneal (i.p.) B-16 melanoma tumors after inoculation of viable tumor cells. More than 40% of prevaccinated animals challenged i.p. with 5x10 power on 5 viable tumor cells were completely protected from tumor development and remained tumor-free 100 days after tumor cell inoculation. The percentage of tumor-free animals (survivors) rose to as much as 90% when the application of tumor vaccine was repeated two weeks after the first vaccination (i.e. one week after the inoculation of viable tumor cells). The induced antitumor response depended predominantlt upon macrophage function, since vaccinated animals which were depleted of peritoneal macrophages died within the same time range as animals in the control group. Also, tumor-type speciificity of the vaccine was confirmed by the fact that the animals vaccinated with B-16 melanoma vaccine were not protected from the development of another type of tumor. In cocnsluion, comparison of the experimental data with the data from the literature suggests that our simple tumor vaccine may be as effective as genetically endineered tumor vaccines. At the same time, this kind of vaccine is easier to control and thus safer to apply in humans when compared to genetically engineered vaccines.
Deskriptorji		MELANOMA TUMOR CELLS, CULTURED CANCER VACCINES MICE, INBRED BALB C