Avtor/Urednik		Krejci, Eric; Legay, Claire; Thomine, Sebastien; Sketelj, Janez; Massoulie, Jean
Naslov		Differences in expression of acetylcholinesterase and collagen Q control the distribution and oligomerization of the collagen-tailed forms in fast and slow muscles
Tip		članek
Vir		J Neurosci
Vol. in št.		Letnik 19, št. 24
Leto izdaje		1999
Obseg		str. 10672-9
Jezik		eng
Abstrakt		The collagen-tailed forms of acetylcholinesterase (AChE) are accumulated at mammalian neurovascular junction. The A4, A8, and A12 forms are expressed differently in the rat fast and slow muscles; the sternomastoid muscle contains essentially the A12 form at end plates, whereas the soleus muscle also contains extrajunctional A4 and A8 forms. We show that collagen Q(ColQ) transcrips become exclusively junctional in the adult sternomastoid but remain uniformly expressed in the soleus. By coinjecting Xenopus oocyted with AChE, and ColQ mRNAs, we reproduced the muscle patterns of collagen0tailed forms. The soleus contains transcripts ColQ1 and ColQ1a, whereas the sternomastoid only contains ColQ1a. Collagen-tailed AChE represents the first evidence that synpatic components involved in cholinergic transmission may be differently regulated in fast and slow muscles.
Deskriptorji		ACETYLCHOLINESTERASE COLLAGEN NEUROMUSCULAR JUNCTION MUSCLE FIBERS, FAST-TWITCH MUSCLE FIBERS, SLOW-TWITCH RATS IN SITU HYBRIDIZATION XENOPUS TRANSCRIPTION, GENETIC OOCYTES RNA, MESSENGER