Avtor/Urednik		Bavec, A; Jureus, A; Cigić, B; Langel, U; Zorko, M
Naslov		Peptitergent PD1 affects the GTPase activity of rat brain cortical membranes
Tip		članek
Vir		Peptides
Vol. in št.		Letnik 20, št. 2
Leto izdaje		1999
Obseg		str. 177-84
Jezik		eng
Abstrakt		Peptitergent PD1 shows complex effects on GTPase activity of rat brain cortical membranes: inhibition in the presence of lower concentrations of GTP and activation at a higher concentration, above 0.5 microM, of GTP. Its effect is dose dependent and is characterized by an EC50 of 1.8 +/- 0.2 microM and a Hill coefficient of 1.6 +/- 0.3, and it increases both Km and Vmax of the GTP hydrolysis. PD1 that was unable to solubilize G-proteins from the membranes probably acts on them by direct binding near the C-terminal alpha-helical region of the Galpha subunit, similarly to mastoparan.
Deskriptorji		CEREBELLAR CORTEX DETERGENTS GTP PHOSPHOHYDROLASE MEMBRANE PROTEINS PEPTIDES AMINO ACID SEQUENCE GUANOSINE TRIPHOSPHATE GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE) HYDROLYSIS MICELLES MODELS, CHEMICAL MOLECULAR SEQUENCE DATA PROTEIN STRUCTURE, SECONDARY RATS, WISTAR SOLUBILITY WASP VENOMS