Avtor/Urednik | Benedičič, Desimira; Ravnikar, Maja; Kovač, Maja | |
Naslov | Auxins in bean (Phaseolus vulgaris L. Cv. Zorin) seedlings | |
Tip | članek | |
Vir | Phyton-Ann Rei Bot A | |
Vol. in št. | Letnik 39, št. 3 Spec Iss | |
Leto izdaje | 1999 | |
Obseg | str. 79-84 | |
Jezik | eng | |
Abstrakt | The amount and composition of auxins were investigated in bean seedlings (Phaseolus vulgaris L.) cv. Zorin. Extracts of cotyledon, leaves, stems with shoots and roots were purified with Sep-Pak C18 cartridges and analysed by HPLC with fiuorescent and photodiode array detectors. Biological activity was determined with an Avena bioassay. The highest amount of indole-3-acetic acid (IAA) was detected in roots. In all analysed tissues bound IAA predominated. HPLC and bioassay confirmed IAA conjugates after hydrolysis with 7 N NaOH. In been seedlings, in addition to free IAA, some conjugates also showed biological activity. | |
Deskriptorji | LEGUMES SEEDS AUXINS GERMINATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID INDOLEACETIC ACIDS COTYLEDON |