Avtor/Urednik | Petrovec, Miroslav | |
Naslov | Erlihioza v Sloveniji: molekularno - epidemiološke značilnosti | |
Prevedeni naslov | Erlichiosis in Slovenia: molecular and epidemiological characteristics | |
Tip | monografija | |
Kraj izdaje | Ljubljana | |
Založnik | Biotehniška fakulteta | |
Leto izdaje | 1999 | |
Obseg | str. 93 | |
Jezik | slo | |
Abstrakt | Tick-transmitted diseases represents important public health problems in Slovenia. During the last decade, two previously unknown human diseases caused by Ehrlichia species have emerged in United States. During this study febrile ilnesses following a tick bite in patients from Slovenia were evaluated for an ehrlichial etiology. With molecular and serological methods we have confirmed first patients infected with granulocytic ehrlichia outside the United States. Among 130 evaluated patients included in prospective study we have found four acute cases of human granulocytic ehrlichiosis. We suggests that human granulocytic ehrlichiosis should be included in differrential diagnosis when evaluating patients with febrile illness after a tick-bite. By using PCR method we have successfully amplified two target regions of ehrlichiae in human patients, deer (Capreolus capreolus), red deer (Cervus elaphus), chamois (Rupicapra rupicapra), and small mammals (Chlethrionomys glareoulus, Apodemus flavicollis and Apodemus sylvaticus). PCR products were sequenced and sequences compared with those of known ehrlichiae. Comparison of GroESL operon sequences revealed that sequences from Slovenian patients were more similar to the sequences obtained from animals in Europe than to sequences amplifed from patients in the United States. In Ixodes ricinus ticks we have indentified sequences 100 % identical with those amplified from patients. Therefore we conclude that I. ricinus is potential vector of granulocytic ehrlichia in Slovenia. In the first study in Slovenia the prevalence of infection among 906 Ixodes ricinus ticks was 9,2 % and 7,2 % for adults and nymphs, respectively. Phylogenetic analysis revealed that granulocytic ehrlichia amplified from patients has 100 % identical sequence in 16S rDNA with prototype human granulocytic ehrlichia from United States. (Abstract truncated at 2000 characters.) | |
Deskriptorji | EHRLICHIOSIS EHRLICHIA IXODES DISEASE VECTORS DEER MURIDAE POLYMERASE CHAIN REACTION GROEL PROTEIN BASE SEQUENCE PHYLOGENY FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT PROSPECTIVE STUDIES RNA, RIBOSOMAL, 16S SERODIAGNOSIS |