Avtor/Urednik		Smrkolj, Tomaž
Naslov		Oblika eritrocita in njegova deformabilnost pri spremenjenih zunanjih pogojih
Prevedeni naslov		Erythrocyte shape and deformability due to variations in external factors
Tip		članek
Vir		Med Razgl
Vol. in št.		Letnik 39, št. 1
Leto izdaje		2000
Obseg		str. 23-31
Jezik		slo
Abstrakt		Changes in erythrocyte shape are necessary in the circulation of blood, therefore deformability is one of the basic properties of erythrocytes. A research project was conducted in order to determine the role of the submembrane protein skeleton in the mechanical stability of erythrocytes. Our hypothesis suggested that changes in the mechanical properties of the protein skeleton should result in changed deformability of the erythrocyte membrane. The properties affecting the deformability of erythrocytes were determined by observing changes in erythrocyte shape resulting from variations in external factors. Changes in erythrocyte shape were induced using sodium salicylate, which transforms biconcave discs (the physiological shape of the erythrocytes) into echinocytes, i. e. spheres with numerous membrane spicules. Diamide was added to strengthen the submembraneous protein skeleton and shape changes resulting from the addition of sodium salicylate were observed again. The results suggest that diamide lowers the deformability of the erythrocyte membrane. The degree of decrease in deformability depends on diamide concentration. Membrane deformability depends on the mechanical characteristics of the submembrane protein skeleton. Lower deformability is the result of higher skeleton strength.
Izvleček		Med pretakanjem krvi morajo eritrociti stalno spreminjati svojo obliko, zato je njihova deformabilnost ena njihovih osnovnih lastnosti. V raziskavi smo skušali ugotoviti vlogo podmembranskega beljakovinskega ogrodja pri mehanski stabilnosti eritrocitov. Predvidevali smo, da bodo spremenjene mehanske lastnosti ogrodja vplivale na deformabilnost membrane eritrocita. Membranske lastnosti, ki pogojujejo deformabilnost eritrocitov, smo zasledovali z opazovanjem spremembe oblike, ki jo eritrociti zavzamejo ob spreminjanju zunanjih pogojev. Za spreminjanje oblike eritrocitov smo uporabili natrijev salicilat, ki spremeni bikonkavni disk, to je fiziološka oblika eritrocita, v ehinocit-kroglo s številnimi membranskimi izrastki. Nato smo utrdili podmembransko beljakovinsko ogrodje z diamidom in ponovno opazovali spremembo oblike ob dodatku natrijevega salicilata. Rezultati so pokazali, da diamid zmanjša deformabilnost eritrocitove membrane. Zmanjšanje je odvisno od koncentracije diamida. Deformabilnost membrane je določena z mehanskimi lastnostmi podmembranskega beljakovinskega ogrodja. Zmanjšanje deformabilnosti je posledica utrditve ogrodja.
Deskriptorji		ERYTHROCYTES ERYTHROCYTE DEFORMABILITY BAND 3 PROTEIN ERYTHROCYTE MEMBRANE SODIUM SALICYLATE DIAMIDE