Avtor/Urednik		Jeruc, Jera; Jurčić, Vesna; Vizjak, Alenka; Hvala, Asta; Babič, Nika; Kveder, Rado; Praprotnik, Sonja; Ferluga, Dušan
Naslov		Tubulo-interstitial involvement in lupus nephritis with emphasis on pathogenesis
Tip		članek
Vir		Wien Klin Wochenschr
Vol. in št.		Letnik 112, št. 15-16
Leto izdaje		2000
Obseg		str. 702-6
Jezik		eng
Abstrakt		Glomerular lesions in lupus nephritis have been extensively studied in recent decades, but much less attention has been paid to the tubulo-interstitial compartment. The aim of this study was to contribute to the understanding of the pathogenesis of tubulo-interstitial lesions in lupus nephritis by analysing their incidence, character, and their associations. One hundred and ninety kidney biopsies of 190 patients fulfilling American Rheumatology Association (ARA) criteria of systemic lupus erythematosus (SLE) were examined by traditional light, immunofluorescence and electron microscopy. Interstital inflammatory infiltration and tubulointerstital immune deposits concurred in 72 cases ((37.9%). Their frequency was the highest in WHO class IV lupus glomerulonephritis. By multivariate analysis, the intensity of interstital inflammatory infiltration correlated best with the percentage of renal corpuscules with extracapillary crescents and the extent of interstitial fibrosis. On immunohistochemical assessment, the inflammatory infiltrate was found to be composed of CD45RO positive T Iymphocytes (191.3/mm2), CD68 positive macrophages (101.7/mm2) and CD45RA positive B Iympocytes (17.2/mm2). For all cell types the median value was higher in cases with extracapillary crescents, and did not correlate with presence and intensity of tubulo-interstital immune deposits. Infiltration showed the tendency of periglomerular distribution, especially around glomeruli showing extracapillary proliferation and destruction of the capsular basal membrane. Rare S100 positive cells were only found in the interstitium. Tubulo-interstital lesions estimated semiquantitatively correlated with the degree of proteinuria. Our findings suggest that tubulo-interstitial deposits do not play a major role in the pathogenesis of tubulo-interstitial lesions. (Abstract truncated at 2000 characters.)
Deskriptorji		LUPUS NEPHRITIS IMMUNE COMPLEX DISEASES BIOPSY WORLD HEALTH ORGANIZATION IMMUNOHISTOCHEMISTRY PEROXIDASE ANTIBODIES, MONOCLONAL FLUORESCENT ANTIBODY TECHNIQUE ANTIGENS, CD45