Avtor/Urednik		Wozniak, Gordana; Trontelj, Peter; Rupnik, Maja
Naslov		Genomic relatedness of Clostridium difficile strains from different toxinotypes and serogroups
Tip		članek
Vir		Anaerobe
Vol. in št.		Letnik 6, št. 4
Leto izdaje		2000
Obseg		str. 261-7
Jezik		eng
Abstrakt		Clostridium difficile strains can be divided into sixteen toxinotypes (0 and I to XV) according to changes in their toxin genes. To detrmine the genomic similarity between toxinotypes, two molecular typing techniques were used, AP-PCR and PFGE. Strains were selected from five serogroups (A1, A15, E, F, X) and represented non-toxinogenic isolates, strains with toxin genes identical to the reference C. difficile strain, VPI 10463 (toxinotype 0), and strains with variant toxin genes from toxinotypes III, IV, V, VI, VIII, IX and XI. The strains studied formed three main clusters, which correlated well serogroups: in the first were strains from serogroup A15 and E; in the second serogroup A1 strains; and in the third, strakns from serogroups F and X. Within these three clusters strains of a single toxinotype were grouped together. Toxinotypes III, IV and VIII were more similar to strains with ordinary toxin genes or non-toxinogenic isolates within the same serogroup than to other toxinotypes. Toxinotypes V, VI, VII and XI, which exhibit similar changes in their toxin genes, seem to be more closely related one to another than to other toxinotypes. It can be concluded that variant Clostridium difficile strains do not have a common ancestor and that groups of different toxinotypes arose independently from strains with ordinary toxin genes.
Deskriptorji		CLOSTRIDIUM DIFFICILE BACTERIAL TOXINS GENOME, BACTERIAL POLYMERASE CHAIN REACTION ELECTROPHORESIS, GEL, PULSED-FIELD