Avtor/Urednik		Koraka, P; Avšič-Županc, T; Osterhaus, ADME; Groen, J
Naslov		Evaluation of two commercially available immunoassays for the detection of hantavirus antibodies in serum samples
Tip		članek
Vir		J Clin Virol
Vol. in št.		Letnik 17, št. 3
Leto izdaje		2000
Obseg		str. 189-96
Jezik		eng
Abstrakt		Background: hantaviruses are members of the family Bunyaviridae and the spectrum of clinical symptoms in humans may vary from sub-clinical to severe haemorrhagic fever with with renal syndrome (HFRS) or pulmonary syndrome (HPS). Several serotypes have been described from which at least five are pathogenic to humans. Each serotype has a different animal reservoir and geographical distribution. In the acute phase of the disease the clinical diagnosis may be confirmed by serology or by polymerase-chain reaction (PCR). Objective: to evaluate two commercially available immunoassays using sera from hantavirus suspected and non-hantavirus patients: an enzyme immunoassay (EIA) developed by MRL diagnostics, for the detection of immunoglobulins M (IgM) and G (IgG) against several hantavirus serotypes and an indirect immunofluorescence assay (IFA) from Progen, based on slides coated with Hantaan virus (HNTV) and Puumala virus (PUUV), infected cells. Study design: a total of 145 serum samples were used for this study. The serum panel included serum samples from patients suspected of mild (n = 91), severe (n = 10) HFRS and patients with other viral infections (n = 44). Results: the agreement between the MRL EIA and the Progen IFA for the detection of IgM and IgG serum antibodies ranged from 87 to 91%, respectively. In the non-hantavirus group one out of 44 samples was positive by the Progen HNTV IgM IFA, none in the Progen PUUV IFA and two samples in the MRL IgM EIA, resulting in specifities of 98, 100 and 95%, respectively. (Abstract truncated at 2000 characters).
Deskriptorji		HANTAVIRUS HANTAVIRUS INFECTIONS IMMUNOENZYME TECHNIQUES FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT IGM IGG ANTIBODIES, VIRAL SENSITIVITY AND SPECIFICITY