Avtor/Urednik		Fazarinc, G; Golob, Z
Naslov		Histophotometric evaluation of metabolic enzyme activity in pig skeletal muscle fibres
Prevedeni naslov		Histofotometrična ocena aktivnosti presnovnih encimov v skeletnih mišičnih vlaknih pri prašiču
Tip		članek
Vir		Slov Vet Res
Vol. in št.		Letnik 37, št. 1-2
Leto izdaje		2000
Obseg		str. 31-9
Jezik		eng
Abstrakt		Using computerized image analysing system the activities of mitochondrial succinate dehydrogenase (SDH), mitochondrial alpha-glycerophosphate dehydrigenase (alpha-GPD) and alpha-glucan phosphorylase in muscle fibre types I, IIa and IIb were determined. M. longissimus dorsi and m. biceps femoris of nine Duroc boars were analysed. The results of the analysis were compared to the muscle pH1 as an marker of the early post-mortem glycolysis. The positive correlation between pH1 and SDH activity was statistically significant (p < 0.05), while the correlation between the pH1 and alpha-GPD was not confirmed (p < 0,1). The negative correlation was determined between the SDH and a-GPD activity (p < 0,01). The alpha-glucan phosphorylase activity was very unstable. There were no significant differences in the enzyme activity between muscle fibre types, neither any correlation between the pH1 and the enzyme activity. The metabolic profile of the muscle fibres could be evaluated sufficiently only by mitochondrial enzymes activity. The SDH and alpha-GPD activity is stable also on the samples affected with intensive glycolysis. The best results were obtained by the histophotometric analysis of the SDH activity. This enzyme allows clear metabolic differentiation of the muscle fibres and is in correlation with the pH1 of the muscle.
Izvleček		Z računalniškim sistemom za analizo slike smo določali aktivnost mitohondrijske sukcinatdehidrogenaze (SDH), mitohondrijske alpha-glicerofosfatdehidrogenaze (alfa-GPD) in alfa-glukanfosforilaze v skeletnih mišičnih vlaknih tipa I, IIa in IIb. Raziskavo smo opravili na vzorcih najdaljše hrbtne (m. longissimus dorsi) in dvoglave stegenske mišice (m. biceps femoris) pri 9 prašičih pasme durok. Rezultate analize aktivnosti presnovnih encimov smo primerjali z vrednostjo pH1 kot kazalcem intenzivnosti glikolize v vzorčenih mišicah. Ugotovili smo statistično značilno soodvisnost med vrednostjo pH1 in aktivnostjo SDH (p<0,05). Soodvisnost med aktivnostjo alfa-GPD in vrednostjo pH1 je nakazana, vendar statistično ni zanesljivo potrjena (p < 0,1). Aktivnost SDH je obratno sorazmerna z aktivnostjo alfa-GPD (p < 0,01). Aktivnost alfa-glukanfosforilaze v mišičnih vlaknih je zelo nestabilna, saj nismo ugotovili statistično značilnih razlik v aktivnosti encima med mišičnimi vlakni tipa I, IIa in IIb, niti soodvisnosti med vrednostjo pH1 in aktivnostjo encima v vlaknih. Na osnovi rezultatov histofotometrične analize aktivnosti encimov ugotavijamo, da je za presojo presnovnega profila mišic pri prašiču primernejše določanje aktivnosti mitohondrijskih encimov, ki sta stabilnejša ter omogočata analizo tudi na mišičnih vzorcih, v katerih je proces glikolize že stekel. Zanesljive rezultate smo dobili z ugotavljanjem aktivnosti SDH, ki omogoča jasno diferenciacijo mišičnih vlaken glede na presnovno aktivnost, aktivnost encima pa je v soodvisnosti z vrednostjo pH1 mišice.
Deskriptorji		MUSCLE, SKELETAL MUSCLE FIBERS IMAGE PROCESSING, COMPUTER-ASSISTED SUCCINATE DEHYDROGENASE GLYCEROLPHOSPHATE DEHYDROGENASE HISTOCYTOCHEMISTRY GLYCOLYSIS SWINE