Avtor/Urednik		Gomišček, Gregor; Arrigler, Vesna; Gros, Marjan; Zupančič, Mirjana; Svetina, Saša
Naslov		Asymmetrical labeling of giant phospholipid vesicles
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 440, št. 5 Suppl
Leto izdaje		2000
Obseg		str. R51-2
Jezik		eng
Abstrakt		The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50% decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.
Deskriptorji		FLUOROMETRY PHOSPHOLIPIDS MEMBRANES, ARTIFICIAL DITHIONITE MICROSCOPY, FLUORESCENCE