Avtor/Urednik		Jurič, Damjana-Mojca; Čarman-Kržan, Marija
Naslov		Cytokine-regulated secretion of nerve growth factor from cultured rat neonatal astrocytes
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 440, št. 5 Suppl
Leto izdaje		2000
Obseg		str. R96-8
Jezik		eng
Abstrakt		Interactions involved in the regulation of nerve growth factor (NGF) release by inflammatory cytokines: interleukin-1beta (IL-1beta) interleukin-6 (IL-6), tumour necrosis factor-alpha. (TNF-alpha) and transforming growth factor-beta 1 (TGF(beta 1) were examined in rat neonatal cortical astrocytes in primary culture. Exposure of cultured astrocytes to IL-1beta IL-6, and TGF-beta1 recalled in the stimulation of NGF secretion. Treatment of cells for 24 h with IL-1beta (10 U/ml), IL-6 (5 ng/ml) and TGF-beta 1 (5 ng/ml) caused 3-, 1.8-, and 2.8-fold increase in NGF secretion as compared to the control cells. In contrast, TNF-alpha (30 ng/ml) by itself had no stimulatory action on NGF release whereas co-stimulation of astrocytes with IL-1beta and TNF-alpha showed a synergistic interaction. Co-treatment of astrocytes with IL-1beta and TGF-beta 1 increased NGF secretion in an additive way, whereas simultaneous application of IL-1beta and IL-6 resulted in the inhibitory effect on NGF secretion. Our results suggest that interactions between cylokines used cause the stimulation of NGF secreation through different regulatory mechanisms.
Deskriptorji		NERVE GROWTH FACTORS ASTROCYTES INTERLEUKIN-1 INTERLEUKIN-6 TUMOR NECROSIS FACTOR RATS ANIMALS, NEWBORN TRANSFORMING GROWTH FACTOR BETA