Avtor/Urednik		Erdani-Kreft, Mateja; Sterle, Maksimiljan
Naslov		The effect of lamina propria on the growth and differentiation of urothelial cells in vitro
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 440, št. 5 Suppl
Leto izdaje		2000
Obseg		str. R181-2
Jezik		eng
Abstrakt		The effect of lamina propria on the growth and differentiation of the mouse urinary bladder urothelial cells in vitro has been studied by light and electron microscopy using morphological and immunohistochemical methods. Three different types of urothelial cultures were maintained on a porous membrane in serum-free medium for 10 days. Our results showed that urothelial cells in culture types 1 and 2 were organised as the multilayer epithelium showing terminal differentiation, from the porous membrane toward the surface. On the surface of culture type 1 and 2, superficial urothelial cells were highy differentiated. They formed tight junctions and had plaques of as asymmetric unit membrane, a hallmark of terminal differentiation in bladder superficial cells. These cultured superficial cells were also cytokeratin 20 positive. On the contrary, urothelial cells in culture type 3 have shown a limited capacity for survival and have grown as poorly differentiated monolayer. These results revealed that reciprocal intercellular signalling between the lamina propria and urotbelial cells is essential to achieve terminal differentiation of urothelial cells in vitro.
Deskriptorji		UROTHELIUM CELL DIVISION CELL DIFFERENTIATION CULTURE MEDIA MICE BLADDER CELLS, CULTURED IMMUNOHISTOCHEMISTRY