Avtor/Urednik		Zagradišnik, Boris; Kokalj-Vokač, Nadja
Naslov		Hypomethylation of alphoid DNA and classical satellite DNA on chromosome 1, 9, 16 and Y in extraembryonic tissue
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 440, št. 5 Suppl
Leto izdaje		2000
Obseg		str. R190-2
Jezik		eng
Abstrakt		The pattern of DNA methylation can he analyzed on methaphase chromosomes with fluorescein labeled antibodies against 5-methylcytosine. In human extmembryonic tissue lower overall intensity of immunofluorescence in centromeric chromosomal regions correspond to hypomethylation of the DNA when compared with normal human lymphocytes. Pericentromeric regions on chromosomes 1,9,16 and heterochromatin on chromosome Y, which reveal lower levels of immunofluorescence, are rich in classical satellite DNA type 2 and 3. In our experiment methylation-sensitive restriction enzymes, alphoid and classical satellite DNA probes specific for chromosomes 1,9,16 and Y were used. Southern blot analysis on cells from extraembryonic tissue revealed different extent of hypomethylation in different chromommal regions. Our results confirm overall and sequence-specific hypomethylation of DNA in cells from extraembryonic tissue in comparison with somatic cells.
Deskriptorji		CHORIONIC VILLI SAMPLING DNA METHYLATION DNA, SATELLITE KARYOTYPING DNA PROBES LEUKOCYTES CHROMOSOMES, HUMAN, PAIR 1 CHROMOSOMES, HUMAN, PAIR 9 CHROMOSOMES, HUMAN, PAIR 16 Y CHROMOSOME