Avtor/Urednik		Černelč, Peter; Kralj, Jana
Naslov		Koncentracija serumskega trombopoetina med zdravljenjem bolnikov z akutno mieloblastno levkemijo s citostatiki
Prevedeni naslov		Serum thrombopoietin levels in patients with acute myeloid leukemia during chemotherapy
Tip		članek
Vir		Med Razgl
Vol. in št.		Letnik 39, št. Suppl 5
Leto izdaje		2000
Obseg		str. 39-42
Jezik		slo
Abstrakt		To understand the regulation of circulating platelet counts (PC) with thrombopoietin (TPO) serum TPO levels and PC before and after myelosuppressive chemotherapy and after the administration of platelet transfusions in 10 patients with acute myeloid leukaemia (AML) were studied. Serum TPO levels were measured by the quantitative sandwich enzyme-linked immunosorbent assay (Quantikine, RD Systems). The lower detection limit of the TPO assay was 30 pg/mL. The median serum TPO level in normal subjects was 55 pg/mL (range 31-88 pg/mL). At the start of induction chemotherapy, the patients had a median serum TPO level of 261 pg/mL (range 120-2150 pg/mL);10 to 12 days after the end of chemotherapy, their TPO levels were substantially increased, the median value being 1900 pg/mL (range 1050-4194 pg/mL). As a result of chemotherapy, the patients developed aplasia; after the administration of platelet transfusions their median PC increased to 21 x 10(9)J1 (range 5-55 x 109/1), while the median TPO value decreased by 300 pg/mL (range 11-1125 pg/mL). The correlation between PC and TPO was statistically significant prior to chemotherapy (p = 0,04). After chemotherapy, in the period of regeneration, PC increased, while TPO levels decreased to normal levels.
Izvleček		Izhodišča. Proučevali smo koncentracijo trombopoetina (TPO) v periferni venski krvi pred zdravljenjem s citostatiki in po njem in pred transfuzijo koncentriranih trombocitov ter po njej pri desetih bolnikih z akutno mieloblastno levkemijo (AML). Rezultati. TPO smo določali kvantitativno na imunoencimski način (Quantikine, RD Systems). Vrednosti TPO v serumu zdravih odraslih oseb so 55 pg/mL (mediana), 31-88 pg/mL. Pri 10 bolnikih z AML so bile vrednosti TPO v serumu pred začetkom zdravljenja s citostatiki 261 pg/mL (mediana), 120-2150 pg/mL; 10-12 dan po zdravljenju, v obdobju najhujše pancitopenije, pa 1900 pg/mL (mediana), 1050-4194 pg/mL. V obdobju aplazije AML po zdravljenju s citostatiki so bolniki prejemali transfuzije koncentriranih trombocitov, v povprečju 6 enot vsak drugi dan. Po transfuziji koncentriranih trombocitov je število trombocitov v venski krvi (Tr) porastlo za 21 x 109/1 (mediana), 5-55 x 10(9)/l, TPO v serumu pa se je zmanjšal za 300 pg/mL (mediana), 11-1125 pg/mL. Zaključki. Povezanost med TPO v serumu in Tr je bila pred zdravljenjem s citostatiki statistično značilna (p = 0,04). 2-3 tedne po zdravljenju s citostatiki se je pri bolnikih z AML, kjer smo dosegli popolno remisijo, koncentracija TPO v serumu zmanjšala na normalno.
Deskriptorji		LEUKEMIA, MYELOCYTIC, ACUTE THROMBOPOIETIN PLATELET COUNT PLATELET TRANSFUSION