Avtor/Urednik		Ocepek, M; Pate, M
Naslov		Species and antigenic structure of mycobacteria, isolated from swine in Slovenia in the years 1996 and 1997
Prevedeni naslov		Vrste in antigenska zgradba mikrobakterij, izoliranih pri prašičih v Sloveniji v letih 1996 in 1997
Tip		članek
Vir		Slov Vet Res
Vol. in št.		Letnik 37, št. 3
Leto izdaje		2000
Obseg		str. 125-32
Jezik		eng
Abstrakt		We have investigated 222 strains of mycobacteria isolated in the years 1996 and 1997. 220 strains were isolated from swine, one from bedding material and one strain from foodstuff. The research work took place in the Laboratory for Mycobacteria, Institute of Microbiology and Parasitology of the Veterinary Faculty in Ljubljana. Most of isolated strains (189 strains) belonged to M. avium complex, 3 strains to M. gordonae, 2 strains to M. fortuitum and 1 strain to M. terrae. 27 strains remained undetermined due to the contamination of the samples. The strains that belonged to M. avium complex were serologically typed. The predominant serotypes, isolated from swine from both large and small farms, were serotypes 8 and 4. Out of 189 strains, 10 strains (5.29 %) belonged to serotype 1, 18 (9.52 %) to serotype 2, 10 (5.29 %) to serotype 3, 23 (12.17 %) to double serotype 2/3, 27 (14.28 %) to serotype 4, 4 (2.12 %) to serotype 6, 30 (15.88 %) to serotype 8, 14 (7.41 %) to serotype 9 and 7 (3.70 %) to mixed serotype 2/7/9. Serotyping failed in 46 cases. Those strains were then typed by polymerase chain reaction (PCR). The results of both typing methods show that 72 (38.10 %) strains belonged to M. avium type A/I, 95 (50.26 %) strains to M. avium type A, 22 (11.64 %) strains were nontypable. The predominant species of mycobacteria in swine in Slovenia are those of M. avium complex. Within the complex, the most frequent serotypes were those of M. avium type A (not pathogenic for poultry) regardless of the system of breeding. However, there was a higher percentage of M. avium type A/I serotypes (poultry pathogens) discovered in strains, isolated from pigs from small farms than in strains, isolated from pigs from large farms.
Izvleček		V letih 1996 in 1997 je bilo v laboratoriju za mikobakterije Inštituta za mikrobiologijo in parazitologijo VF v Ljubljani izoliranih 220 sevov mikobakterij iz organov prašičev, en sev iz žagovine, ki se je uporabljala za nastil v manjši reji prašičev, in en sev iz travne silaže, ki se je uporabljala za krmo v isti reji. V 189 primerih so bile izolirane mikobakterije sklopa M. avium, 3x M. gordonae, 2x M. fortuitum in lx M. terrae. Zaradi kontaminacije vzorcev določitev vrste ni bila uspešna v 27 primerih. Od skupno 189 serološko tipiziranih sevov mikobakterij sklopa M. avium jih je največ pripadalo serovaroma 8 in 4. Serovar 8 je bil ugotovljen v 30 primerih (15,88 %), serovar 4 pa v 27 (14,28 %). Dvojni serovar 2/3 je bil ugotovljen v 23 primerih (12,17 %), serovar 2 v 18 (9,52 %), serovar 9 v 14 (7,41%), serovara 1 in 3 v 10 primerih (5,29 %), mešani serovar 2/7/9 v 7 (3,70 %) ter serovar 6 v 4 primerih (2,12 %). V 46 primerih serološka tipizacija ni bila uspešna. Ti sevi so bili dodatno tipizirani s polimerazno verižno reakcijo (PCR). Skupni rezultati tipizacij so naslednji: 72 (38,10 %) sevov je pripadalo M. avium tipa A/I, 95 (50,26 %) M. avium tipa A, pri 22 sevih (11,64 %) pa tipizacija ni bila uspešna. Pri prašičih v Sloveniji so v letih 1996 in 1997 prevladovale mikobakterije sklopa M. avium. Znotraj sklopa so bili najpogostejši serovari, ki niso patogeni za perutnino (M. avium tipa A). Kljub temu da so ti serovari prevladovali ne glede na način reje, je bil v manjših rejah večji delež serovarov M. avium, patogenih za perutnino (M. avium tipa A/I) večji kot pri prašičih iz večjih rej.
Deskriptorji		MYCOBACTERIUM MYCOBACTERIUM AVIUM COMPLEX SWINE MYCOBACTERIUM AVIUM SEROTYPING POLYMERASE CHAIN REACTION