Avtor/Urednik		Bajrović, Fajko
Naslov		Vpliv celične podpore v distalnem krnu na regeneracijo senzoričnih aksonov perifernega živca pri podgani
Prevedeni naslov		Influence of cell support in the distal stump on regeneration of sensory axons of peripheral nerve in rat
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2000
Obseg		str. 146
Jezik		slo
Abstrakt		Growth of regenerating axons after peripheral nerve injury is determined predominantly by the micro-environment in the nerve segment distally to the lesion site. It is still contrversial as to what extent the proliferating Schwann cells (SC) in the distal stump support axon growth. If a frozen nerve graft containing basal laminae (BL) but not SCs was sutured to the proximal stump after nerve transection, the outgrowth of regenerating axons was slow and proceeded in parallel with the SC migrating from the proximal nenre stump. On the other hand, regenerating axons were able to grow fairly rapidly into the acellular distal nerve segment after crush injury, which leaves the BL tubes undamaged. The aim of the present work was to determine the role of cell support in the distal nerve stump on sensory axon regeneration after peripheral nerve injury. We hypothesized that: 1) Recovery of nociception in the skin after peripheral nerve crush is delayed but not prevented by short-term deprivation of cell support in the distal stump. 2) The rate of elongation of regenerating sensory axons after the nerve crush is greatly decreased by long-term deprivation of cellular support in distal nerve stump. In parallel the possibility that prior exposure of sensory neurons to high levels of NGF during collateral sprouting into denervated skin migh compensate for the loss of cell support during subsequent injury and regeneration. Therefore, branches of the rat sciatic nerve were crushed and the nerve segment distally to the lesion site was frozen and thawed to eliminate SC, but leave their BL undamaged. The recovery of nociception in the rats hind paw was tested by skin pinch test. The elongation rate of sensory axons regenerating in the absence of cellular support in the long segment of the distal stump was examined by the nerve pinch test and axon counting. (Abstract truncated at 2000 characters)
Deskriptorji		SCIATIC NERVE NERVE CRUSH NERVE REGENERATION AXONS NERVE GROWTH FACTORS NOCICEPTORS PAIN MEASUREMENT IMMUNOHISTOCHEMISTRY RATS