Avtor/Urednik		Marin, Irena J; Poljak, Mario; Seme, Katja; Meglič-Volkar, Jelka; Matičič, Mojca; Lešničar, Gorazd; Brinovec, Vladimir
Naslov		Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR test and manual microwell plate-based AMPLICOR HBV MONITOR test
Tip		članek
Vir		J Clin Microbiol
Vol. in št.		Letnik 39, št. 2
Leto izdaje		2001
Obseg		str. 758-61
Jezik		eng
Abstrakt		Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV UNA positive by both assays. Three sampfes tested positive by COBASHBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related (n = 183, r = 097, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.
Deskriptorji		HEPATITIS B VIRUS DNA, VIRAL POLYMERASE CHAIN REACTION ENZYME-LINKED IMMUNOSORBENT ASSAY REAGENT KITS, DIAGNOSTIC