Avtor/Urednik		Mlinarič, Aleš; Kreft, Samo; Umek, Andrej; Štrukelj, Borut; Popovič, Tatjana
Naslov		Cysteine proteinase inhibitors screening of fungal species growing in Slovenia
Prevedeni naslov		Iskanje inhibitorjev cisteinskih proteinaz v glivah s področja Slovenije
Tip		članek
Vir		Acta Pharm Zagreb
Vol. in št.		Letnik 50, št. 1
Leto izdaje		2000
Obseg		str. 39-48
Jezik		eng
Abstrakt		Ninety extracts obtained from fungi growing in Slovenia were screened for cysteine proteinase inhibitory activity using in vitro assay. Papain, human cathepsin B and human cathepsin H were the three enzymes used in this study. Our results suggest that at least two fungi from all the samples examined might contain substances with inhibitory activity. One fraction of the extract obtained from Cortinarius violaceus (L. Fr.) Fr. (Basidiomycotina) and two fractions of the extract obtained from Gloeophyllum odoratum (Wulf. Fr.) Imaz. (Basidiomycotina) exhibited the highest inhibitory activity and were selected for further investigation.
Izvleček		Izvlečkom iz devetdesetih vrst gliv s področja Slovenije smo v okviru naše raziskave s pomočjo in vitro testnega sistema preizkusili inhibitorno aktivnost na cisteinske proteinaze. Izbrane proteinaze so bile papain, humani katepsin B in humani katepsin H. Ugotovili smo, da bi vsaj dve glivi lahko vsebovali učinkovine z inhibitornim delovanjem. Najvišjo inhibitorno aktivnost smo zasledili pri eni frakciji izvlečka glive Cortinarius violaceus (L. Fr.) Fr. (Basidiomycotina) in pri dveh frakcijah izvlečka glive Gloeophyllum odoratum (Wulf. Fr.) Imaz. (Basidiomycotina). Omenjene frakcije so predmet nadaljnih raziskav.
Deskriptorji		BASIDIOMYCETES CYSTEINE PROTEINASE INHIBITORS ASCOMYCETES SLOVENIA CATHEPSINS PAPAIN CHROMATOGRAPHY