Avtor/Urednik		Sočan, M; Keše, D; Marinič-Fišer, N
Naslov		Polymerase chain reaction for detection of legionellae DNA in urine samples from patients with community-acquired pneumonia
Tip		članek
Vir		Folia Microbiol (Praha)
Vol. in št.		Letnik 45, št. 5
Leto izdaje		2000
Obseg		str. 469-72
Jezik		eng
Abstrakt		Polymerase chain reaction (PCR) was used for detecting Legionella DNA in water, sputum, tracheal aspirate and bronchoalveolar lavage fluid. There is paucity of data on the use of PCR for detection of Legionella in serum and urine samples. In 82 patients admitted with community-acquired pneumonia, urinary PCR was used in addition to urinary antigen assay for Legionella pneumophila serogroup 1 and serological tests (indirect immunofluorescence and ELISA) in paired sera. PCR was positive in urine samples from 21 patients (26%): in six of seven patients with acute legionellosis by CDC criteria and 15 patients with negative urine antigen showing no fourfold rise in antibody titers in immunofluorescence test.
Deskriptorji		PNEUMONIA, BACTERIAL COMMUNITY-ACQUIRED INFECTIONS LEGIONELLA PNEUMOPHILA POLYMERASE CHAIN REACTION DNA, BACTERIAL FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY