Avtor/Urednik		Lanišnik-Rižner, Tea; Adamski, Jerzy; Žakelj-Mavrič, Marija
Naslov		Expression of 17beta-hydroxysteroid dehydrogenases in mesophilic and extremophilic yeast
Tip		članek
Vir		Steroids
Vol. in št.		Letnik 66, št. 1
Leto izdaje		2001
Obseg		str. 49-54
Jezik		eng
Abstrakt		17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are enzymes responsible for reversible interconversions of biologically active 17-hydroxy and inactive 17-keto steroids. We have performed a survey of 17beta-HSD activity in yeast. Constitutive 17beta-HSD activity was found in three mesophilic yeast species: Candida tropicalis, Cryptococcus tsukubaensis, and Saccharomyces cerevisiae as well as in three extremophilic black yeast species: Hortaea werneckii, Trimmatostroma salinum, and Phaeotheca triangularis, indicating that 17beta-HSD activity is widely distributed among yeast. In extremophilic black yeast, NaCl modulated enzyme activity. Enzymes resembling 17beta-HSD from the filamentous fungus Cochliobolus lunatus were detected in Trimmatostroma salinum and Phaeotheca triangularis. Sequences with identity to the Saccharomyces cerevisiae YBR159w gene were not observed in other yeast species possessing a similar enzyme activity. The results suggest the existence of at least three different types of 17beta-HSD in yeast.
Deskriptorji		YEASTS 17-HYDROXYSTEROID DEHYDROGENASES GENE EXPRESSION REGULATION, ENZYMOLOGIC SEQUENCE HOMOLOGY, NUCLEIC ACID SODIUM CHLORIDE SACCHAROMYCES CEREVISIAE