Avtor/Urednik		Snoj-Cvetko, Erika
Naslov		Histokemične in morfometrične značilnosti mišice vastus lateralis pri otrocih
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1992
Obseg		str. 54
Jezik		slo
Abstrakt		The autopsy samples of vastus lateralis muscle in 50 healthy children who had suffered sudden death were analyzed. The age range was from 14 days to 15 years. On muscle samples of 36 subjects, combined staining for myofibrillar adenosinetriphosphatase (ATPase) and diaphorase I or calcium method staining for myofibrillar ATPase at pH 9.4 were performed. Fibres of types I and II were demonstrated. Fibre types I, IIA, IIB and IIC were presented after performance of calcium method for myofibrillar ATPase at pH 9.4,4.6 and 4.3 on succesive cross-sections of muscle samples of 14 subjects. A computer-assisted method was applied to photographs of cross-sections. Numerical density (NN) and spatial distribution of fibre types were calculated in all muscle samples. In 44 muscle samples, areal density (AA) and mean diameter (D) were determined. On cross-sections of 14 samples spatial arrangement of fibre types and subtypes in particular layers within fascicle were determined. In the age range from 14 days to 1 year, low numerical density of type I fibres 32.3 %) was observed; it increased to statistically significant 59.15 % up to 3 years of age and remained constant in the age group of up to 10 years. In the age range from 10 to 15 years, numerical density was reduced to statistically significant 44.19 %. In type II fibre group, IIA fibres predominated (46.7 % of whole fibre types population) in the first year of life. In the age range from 6 to 8 years numerical density of IIA and IIB fibres was equal (18 %). IIC fibres, however, were rare and could be demonstrated only to the age of 8 years. In the whole life period fibres are arranged randomly on a cross plane of muscles. There is a preponderance of type I fibres inside the fascicle and on the periphery of type II fibres. In type II fibre group, a high proportion of IIB fibres was in the first layer of fasciculum whereas IIA fibres were distributed rather uniformly in all layers. (Abstract truncated at 2000 characters)
Deskriptorji		MUSCLE, SKELETAL MUSCLE FIBERS CHILD MYOSIN ATPASE HISTOCYTOCHEMISTRY