Avtor/Urednik		Werle, Bernd; Kraft, Clemens; Lah, Tamara T; Kos, Janko; Schanzenbaecher, Ulrike; Kayser, Klaus; Ebert, Werner; Spiess, Eberhard
Naslov		Cathepsin B in infiltrated lymph nosed is of prognostic significance for patients with nonsmall cell lung carcinoma
Tip		članek
Vir		Cancer
Vol. in št.		Letnik 89, št. 11
Leto izdaje		2000
Obseg		str. 2282-91
Jezik		eng
Abstrakt		Background.Tumor cells require specific proteolytic enzymes for invasion and metastasis, including lysosomal peptidases-cathepsins. Cathepsin B is a lysosomal cysteine peptidase, which appears to play a major role in invasion and metastasis of human tumors. In this study, the authors focused on the possible role of cathepsin B in lymphogenic metastasis by investigating the enzyme localization and its activity in lung tumors,and corresponding tumor-infiltrated lymph nodes. Methods. Cathepsin B activity was determined in lung tumors, lung parenchyma, and tumor cell-infiltrated and noninfiltrated regional lymph nodes of the same patient. The authors investigated 35 cancer patients suffering from nonsmall cell lung carcinoma. Cathepsin B throughout activity was measured by cleavage of the fiuorogenic substrate Z-Arg-Arg-AMC at pH 6.0. Results. The median specific cathepsin B activity was highest in tumors, followed by the infiltrated lymph nodes, noninfiltrated lymph nodes, and lung parenchyma. The authors showed a significant 1.8-fold increase in cathepsin B activity in tumor-infiltrated lymph nodes compared with noninfiltrated regional lymph nodes and a 4.5-fold increase in lung tumor tissue compared with lung parenchyma. High cathepsin B activity, both in tumors and tumor cell-infiltrated lymph nodes, indicated poor prognosis for overall survival. Immunohistochemical analysis showed the presence of cathepsin B in histiocytes and tumor cells but not in lymphocytes of lymph node tissue. Conclusions. The authors' findings on higher cathepsin B levels in tumor cellinfittrated lymph nodes show that increased level of cathepsin B activity is characteristic of the invasive tumor cell phenotype. This corroborates the hypothesis, that tumor cell associated cathepsin B may play a role in lymphogenic metastasis. The authors' results support the use of lymph node associated cathepsin B as a prognostic factor for survival of patients with lung carcinoma.
Deskriptorji		CARCINOMA, NON-SMALL-CELL LUNG LYMPHATIC METASTASIS CATHEPSIN B PROGNOSIS SURVIVAL ANALYSIS ADENOCARCINOMA CARCINOMA, SQUAMOUS CELL LYMPH NODE EXCISION NEOPLASM STAGING IMMUNOHISTOCHEMISTRY LUNG NEOPLASMS