Avtor/Urednik		Dachs, Gabi U; Čemažar, Maja; Wilson, Ian; Heller, Richard; Heller, Lorre C; Jaroszeski, Mark J; Serša, Gregor; Tozer, Gillian M
Naslov		A comparison of gene delivery methods using electroporation and lipofectin-based techniques in vitro and in vivo
Tip		članek
Vir		In: 11th international conference on Tumor physiology and cancer treatment; 2000 Oct 5-7; Banff, Alberta. Banff: ,
Leto izdaje		2000
Obseg		str. 197-8
Jezik		eng
Abstrakt		The main stumbling block for the gene therapy of cancer remains the delivery of genetic materiat to tumours. Non-viral delivery methods are still plagued by poor transfection efficiencies in vivo. However, the advantages regarding safety and patient confidence, as weil as the possibility of targeted delivery, make non-viral methods attractive for further development. Electric pulses have been used successfully for drug and gene delivery in vitro, and recent in vivo results are promising (Niu et al., 1999, Goto et al., 2000). Antitumour effects of electrochemotherapy have been demonstrated in clinical studies (Sersa ef al., 2000). Lipofectin-based gene delivery is widely used in vifro, and recent in vivo results reported around 1% of tumour cells transfected (Kikuchi et al., 1999). Integrin-targeted delivery has proven very effective in a range of in vifro cell cultures, and recent in vivo results demonstrated transfection efficiencies in healthy rat lungs similar to those achievab(e using adenovirus (Hart et al., 1998, Jenkins et al., 2000). We have compared electroporation, lipofectin and integrin-targeted lipofectin to deliver the marker gene green fluorescent protein (GFP) to cells in vifro and to tumours in vivo. ln vifro experiments in rodent and human tumour cell lines showed a variation in transfecfion efficiency depending on the method and cell line. Integrin-mediated transfection and electroporation were superior with up to 66% and 57% of cells transfected, respectively. Our in vivo data showed that different tumours have different susceptibility to transfection. Electroporation showed a higher efficiency than lipofectin-based methods, with up to 4.8% of ce(Is fransfected in the tumours. Transfection efficiency was very heterogeneous and GFP fluorescence varied both qualitatively and quantitatively between tumour models and treatments. (Abstracts truncated at 2000 characters).
Deskriptorji		NEOPLASMS ELECTROPORATION GENE THERAPY MICE TRANSFECTION