Avtor/Urednik		Comino, Aleksandra; Bedina-Zavec, Apolonija; Manček, Mateja; Komel, Radovan
Naslov		The product of ECM11 gene might couple the cell cycle events with the extracellular matrix composition
Prevedeni naslov		Produkt ECM11 gena verjetno povezuje regulacijo celičnega cikla z regulacijo izgradnje celične stene
Tip		članek
Vir		Acta Biol Slov
Vol. in št.		Letnik 43, št. 1-2
Leto izdaje		2000
Obseg		str. 67-71
Jezik		eng
Abstrakt		Product of ECM11 gene was found to interact with Cdc6 protein used as bait in two-hybrid screen. Among all five positive clones obtained by the screen, it activities both reporter genes the most efficiently. By fusion with green fluorescent protein (GFP) we demonstrated that the gene product Ecm11 is located in the cell nucleus throughout the whole cell cycle. Since all known functions of Cdc6p are associated with chromatin, this result makes the interaction of Cdc6 with Ecm11 credible.
Izvleček		Ugotovili smo, da produkt gena ECM11 aktivira oba poročevalska gena, če smo kot vabo v poskusu dvohibridnih interakcij uporabili Cdc6. Med vsemi petimi kloni, ki smo jih dobili ob pregledovanju genske banke, je bila reakcija vabe Cdc6 s plenom Ecm11 najmočnejša. Protein Ecm11 smo izrazili kot fuzijski protein z zeleno-fluorescirajočim proteinom (GFP) in ugotovili, da se nahaja v jedru v vseh fazah celičnega cikla. Ker so vse znane funkcije proteina Cdc6 vezane na kromatin, obstaja velika verjetnost, da Cdc6 in Ecm11 dejansko interagirata.
Deskriptorji		CELL CYCLE CELL WALL GENES, CDC CELL CYCLE PROTEINS MICROSCOPY, FLUORESCENCE