| Avtor/Urednik | Božič, Mojca; Skitek, Milan; Ritonja, Anka; Jerin, Aleš; Stegnar, Mojca | |
| Naslov | Določanje celokupnega plazemskega homocisteina | |
| Prevedeni naslov | Determination of total plasma homocysteine | |
| Tip | članek | |
| Vir | Farm Vestn | |
| Vol. in št. | Letnik 52, št. 1 | |
| Leto izdaje | 2001 | |
| Obseg | str. 19-24 | |
| Jezik | slo | |
| Abstrakt | Numerous studies performed in recent years have confirmed that elevated total plasma homocysteine (cHCE) presents an independent risk factor for vascular diseases such as peripheral artery occlusive disease, ischemic stroke, myocardial infarction and venous thrombosis. In order to detect subjects who are at a higher risk for vascular disease, increasing demands were placed on laboratories to measure cHCE, which has lead to development of numerous chromatographic and immunoassay methods. The aim of this study was to test different methods for cHCE determination and to asses, which of the methods are suitable for clinical laboratory. We tested two chromatographic methods (amino-acid analyser and high-pressure liquid chromatography with fluorimetric detection) and two immunoassay's (fluorescence immunoassay and enzyme-immuno assay). The characteristics of all tested methods were good. However, interassay variability and technical demands were higher for amino-acid analyser, therefore this method was excluded from further comparison. High-pressure liquid chromatography was in agreement with both immunoassays, whereas systemic bias was observed between the two immunoassays. We conclude, that high-pressure liquid chromatography with fluorimetric detection is suitable for use in a clinical laboratory. Fluorescence immunoassay could substitute this method in laboratories without chromatography equipment, while enzyme-linked immunoassay could be utilised in laboratories with high sample throughput. | |
| Izvleček | V zadnjih letih so številne raziskave potrdile, da je povečana koncentracija celokupnega homocisteina (cHCE) v krvi neodvisni dejavnik tveganja za žilne bolezni, kot so okluzivna bolezen perifernih arterij, ishemični inzult, srčni infarkt in venska tromboza. Vse večje zahteve po določanju cHCE, ki bi omogočilo odkrivanje oseb z večjim tveganjem za žilne bolezni, so vodile do razvoja številnih kromatografskih in imunoloških metod. Namen raziskave je bil preizkusiti različne metode za določanje cHCE in oceniti, katere metode so primerne za uporabo v kliničnem laboratoriju. Izbrali smo štiri metode, in sicer dve kromatografski (aminokislinski analizator in visokotlačno tekočinsko kromatografijo s fluorimetrično detekcijo) in dve imunski (fluorescenčno-imunska in encimsko-imunska) metodi. Lastnosti vseh preizkušenih metod so bile dobre. S slabšo medserijsko ponovljivostjo in večjo tehnično zahtevnostjo je odstopala samo metoda na aminokislinskem analizatorju, ki je zato v nadaljnjo primerjavo nismo vključili. Metoda z visokotlačno kromatografijo se je dobro ujemala z obema imunskima metodama, med katerima pa smo opazili sistemsko odstopanje. Ugotovili smo, da je za klinični laboratorij primerna visokotlačna tekočinska kromatografska metoda s fluorimetrično detekcijo, ki je primerljiva z obema imunskima metodama. Fluorescenčno-imunska metoda je primerna za laboratorije brez opreme za visokotlačno tekočinsko kromatografijo, enclmsko-imunska metoda pa za laboratorije z večjimi serijami vzorcev. | |
| Deskriptorji | HOMOCYSTEINE CHROMATOGRAPHY THROMBOPHLEBITIS PULMONARY EMBOLISM CHROMATOGRAPHY, HIGH PRESSURE LIQUID AMINO ACIDS FLUOROMETRY ENZYME-LINKED IMMUNOSORBENT ASSAY |