Avtor/Urednik		Pucihar, G; Mir, LM; Miklavčič, D
Naslov		The effect of pulse repetition frequency on lucifer yellow uptake by means of cell electropermeabilization in vitro
Tip		članek
Vir		Medicon 2001. 9th Mediterranean conference on medical and biological engineering and computing. 2nd Croatian-Slovenian meeting on biomedical engineering. 1st international conference on muscle fibre structure and function. 5th symposium of Croatian society for medical informatics (Medicine informatics 2001). Proccedings of the International federation for medical and biological engineering. Part 1, Part 2; 2001 Jun 12-15; Pula
Leto izdaje		2001
Obseg		str. 834-6
Jezik		eng
Abstrakt		Electrochemotherapy is a technique where electric pulses in combination with chemotherapeutic agents are applied on tumour cells. In general, patients find electrochemotherapy tolerable, despite unpleasant sensations associated with contraction of muscles located beneath the electrodes. These painful and unpleasant muscle contractions are due to the intensity of the electric pulses required for effective electropermeabilization of tumour cell membranes. Because a train of electric pulses with repetition frequency of 1 Hz is usually applied to the tumours, the muscles of patients are excited with each of the pulse in the train of pulses. Therefore, for patients involved In electrochemotherapy, the use of pulses with repetition frequency higher than the frequency of tetanic contraction would represent reduced pain, less unpleasant sensations, and faster therapy. Our results of the uptake of Lucifer yellow Into electropermeabilized cells fn vitro have shown that increasing the repetition frequency results in a reduced but still acceptable uptake even at frequencies up to 1kHz. These encouraging results represent the first step on the way to the clinical use of pulses with high repetition frequency in electrochemotherapy.
Deskriptorji		ELECTROPORATION CELL LINE, TRANSFORMED FLUORESCENT DYES