Avtor/Urednik     Trkov, M; Accetto, T; Kostanjšek, R; Avguštin, G
Naslov     Preliminary characterization of a tentatively novel rumen bacterial species from the genus Treponema
Tip     članek
Vir     Folia Microbiol (Praha)
Vol. in št.     Letnik 46, št. 1
Leto izdaje     2001
Obseg     str. 91-3
ISSN     0015-5632
Jezik     eng
Abstrakt     A new spirochetal strain was isolated from the rumen of a black-and-white Holstein cow and preliminarily characterized. The sugar fermentation tests and morphological observations indicated this organism to be a member of a novel, as yet undescribed spirochetal rumen species. The small subunit ribosomal RNA genes were amplified arul the PCR products were cut with the restriction endonucfeases Taql, Ddel, Hhal and Sau3Al. The comparison of the observed RFLP with the hypothetical fragment lengths of the computer analyzed 16S rRNA sequences from the type strains of the ruminal spirochetes Treponema brynntii and T. saccharophilum confirmed the tenta tive novel identification. Transmission electron microscopy showed that the bacterium has the typical spirochetal structures, i.e. the outer sheath, the protoplasmic cylinder and the axial filament (it is not yet clear how many flagella compose the filament). An additional extracellular structure was observed which appeared as an exocytoplasmic polar flagellum, approximately 2 microm long and protruding from one tip of the cell. The average size of the cells was 0.5 x 10-15 microm and the wavelengths and the amplitudes of the primary coils were 2.9 and 1.3 pm, respectively.
Deskriptorji     RUMEN
TREPONEMA
CATTLE
RNA, RIBOSOMAL
POLYMERASE CHAIN REACTION