Avtor/Urednik | Poljak, Mario; Seme, Katja | |
Naslov | Molekularni mikrobiološki laboratorij v novem tisočletju | |
Prevedeni naslov | Molecular microbiology laboratory in the new millenium | |
Tip | članek | |
Vir | In: Mueller-Premru M, Gubina M, editors. Mikrobi in antibiotiki 2001. Zbornik predavanj Mikrobiološki simpozij z mednarodno udeležbo; 2001 jun 22-23; Ljubljana. Ljubljana: Slovensko zdravniško društvo, Sekcija za klinično mikrobiologijo in hospitalne infekcije, | |
Leto izdaje | 2001 | |
Obseg | str. 67-72 | |
Jezik | slo | |
Abstrakt | The clinical microbiology must evolve if it is to meet the demands of modem medicine. Automation is the only way for the microbiology laboratory to respond to the rapidly increasing demands for more standardization, uniformity and reliability. Therefore, a number of automated and semiautomated analyzers were introduced in the past five years in the clinical microbiology laboratory. The majority of the current clinical microbiology analyzers detect and characterize microorganisms using different nucleic acid-based technologies. Among them, those based on real-time polymerase chain reaction seem to be the most promising. Microarray technology is another emerging technology which uses small analytic devices known as microchips or oligonucleotide arrays for the detection and characterization of microbial DNA or RNA. Miniaturization, accuracy and speed are the most attractive advantages of using microarrays for molecular analysis. Only minute amounts of sample are required (nl instead of Nmicrol for analysis, reagent consumption is low, and all of the steps in a complex analytic procedure can be integrated on a single microdevice. | |
Deskriptorji | MICROBIOLOGICAL TECHNIQUES MOLECULAR BIOLOGY POLYMERASE CHAIN REACTION DIAGNOSTIC TESTS, ROUTINE DNA, BACTERIAL RNA, BACTERIAL |