Avtor/Urednik		Rozman, Janez; Zorko, B; Bunc, M
Naslov		Recording of electroneurograms from the nerves innervating the pancreas of a dog
Tip		članek
Vir		J Neurosci Methods
Vol. in št.		Letnik 112, št. 2
Leto izdaje		2001
Obseg		str. 155-62
Jezik		eng
Abstrakt		Electroneurograms (ENGs) from the vagus, splanchnic and pancreatic nerves innervating the pancreas of a dog, were recorded with chronically implanted silicone multi-electrode circular cuffs in an intact pancreas and in a pancreas partly disabled with alloxam. The cuffs contained 33 platinum electrodes (0.6 x 1.5 mm) arranged in three parallel circular groups integrated into the inner vsurface of the cuff. Each circular group contained 11 electrodes at a distance of 0.5 mm apart, with 6 mm between the circular groups. The cuffs had an inner diameter of 2.5 mm and the length of 18 mm. In a 2-year study, the cuffs were implanted into two adult Beagle dogs (one female and one male). In the vagus nerve, the cuff was installed on the nerve at the neck, whilst in the splanchnic nerve, the cuff was installed on the nerve before the celiac ganglion, and in the pancreatic nerve, the cuff was installed on the nerve just before it enters the pancreas. In each of the three implanted cuffs, the electrodes of the central circular group were connected to each other and this signal provided one input to a multi-channel ENG amplifying system. The electrodes of eaclh of the two outer spiral groups were connected to each other and then both these groups were short-circuited. This signal then provided another input to the multi-channel ENG amplifying system. The ENG amplifying system was designed to amplify the ENGs 100000 times and to pass frequencies of between 500 and 10 kHz. In our study, three recordings in each animal were conduccted. Recordings in the intact pancreas were conducted 2 and 6 months after the implantation, while the recording in the partlyidisabled pancreas, was conducted 10 months after the implantation and 10 days after the disablement. Due to the fact that the results obtained in both animals were actually quite similar, we present the results of the recordings obtained in one animal. (Abstract truncated at 2000 characters)
Deskriptorji		PANCREAS SPLANCHNIC NERVES VAGUS NERVE ELECTRIC STIMULATION DIABETES MELLITUS DOGS ALLOXAN ELECTRODES, IMPLANTED