Avtor/Urednik		Cotman, Marko; Rozman, Damjana; Banek, Ljerka; Ježek, Davor
Naslov		Localisation of lanosterol 14alpha-demethylase in round and elongated sprematids of the mouse testis: an immunoelectron microscopic and stereological study
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 442, št. 6 Suppl 1
Leto izdaje		2001
Obseg		str. R167-8
Jezik		eng
Abstrakt		Lanosterol 14alpha-demethylase (CYP51) is a microsomal cytochrome P450 enzyme involved in the postsqualene cholesterol biosynthetic pathway. CYP51 removes 14alpha-methyl group from lanosterol), forming FFMAS (folicular fluid meiosis activating sterol) which accumulates in gonads. The goal of our study is to determine the expression of CYP51 protein in the male gonad. Using electron microscopic immunogold techniques, CYP51 is localised on inner and outer acrosomal membranes of male germ cells, the round and elongated spermatids. Significance of CYP51 localization on the acrosome which is a Golgi-derived organelle is not known, but we propose that CYP51-formed FF-MAS can function as a signalling sterol during fertilisation.
Deskriptorji		TESTIS SPERMATIDS CYTOCHROME P-450 MICE IMMUNOHISTOCHEMISTRY