Avtor/Urednik		Kubinova, Lucie; Janaček, Jiri; Ribarič, Samo; Čebašek, Vita; Eržen, Ida
Naslov		Three-dimensional study of the capillary supply of skeletal muscle fibres using confocal microscopy
Tip		članek
Vir		J Muscle Res Cell Motil
Vol. in št.		Letnik 22
Leto izdaje		2001
Obseg		str. 217-27
Abstrakt		Three-dimensional (3D) study of capillary network of individual muscle fibres in rat extensor digitorum longus (EDL) and soleus (SOL) muscles is presented. Stereology and 3D reconstruction techniques were applied to stacks of serial optical sections recorded by a confocal microscope from thick muscle slices. The results suggest that SOL muscle fibres have a larger surface area and volume as well as a larger length of capillaries per fibre length than EDL. On the other hand, these two muscles have a similar ratio of capillary length to fibre surface area. The 3D approach to evaluation of muscle fibre capillarization brings many advantages over traditional measurements made on single muscle sections and could also be applied to the study of angiogenesis in other tissues.
Deskriptorji		MUSCLE FIBERS MUSCLE, SKELETAL CAPILLARIES RATS, WISTAR BIOMETRY IMAGE INTERPRETATION, COMPUTER-ASSISTED