Avtor/Urednik		Erman, Andreja; Jezernik, Kristijan; Štiblar-Martinčič, Draga; Romih, Rok; Veranič, Peter
Naslov		Postnatal restoration of the mouse urinary bladder urothelium
Tip		članek
Vir		Histochem Cell Biol
Vol. in št.		Letnik 115, št. 4
Leto izdaje		2001
Obseg		str. 309-16
Jezik		eng
Abstrakt		Mouse urothelium is disrupted just before birth, followed by a postnatal restoration process which includes cell proliferation, death and differentiation. We assessed urothelial proliferation by the expression of proliferating cell nuclear antigen (PCNA), desquamation by electron microscopy, and apoptosis by TUNEL staining and urothelial differentiation by the expression of uroplakins and cytokeratin 20 (CK20) as well as the apical plasma membrane maturation. Our results indicated that urothelial proliferation was high from birth until about the 14th postnatal day. A majority of basal cells and even occasional superficial cells were PCNA positive during the first 5 postnatal days. Cell death occurred during the first 9 postnatal days. Between birth and day 5, single cells underwent apoptosis, whereas between days 6 and 9 cells mainly desquamated. CK20 and uroplakins were expressed in all superficial cells in postnatal urothelium. Their subcellular distribution characteristically changed in accordance with the progressive differentiation of superficial cells. During the urothelial postnatal development, proliferation activity slowly decreases to the proliferatively quiescent urothelium of the adult animal. Apoptosis is present in the first 9 postnatal days and within a few days of this period it appears simultaneously with desquamation. Superficial urothelial cells gradually differentiate, which is reflected in the changeable morphology of the apical plasma membrane.
Deskriptorji		APOPTOSIS BLADDER UROTHELIUM PROLIFERATING CELL NUCLEAR ANTIGEN MICE CELL DIVISION MICROSCOPY, ELECTRON ANIMALS, NEWBORN HISTOCYTOCHEMISTRY