Avtor/Urednik     Barborič, Matjaž; Taube, Ran; Nekrep, Nada; Fujinaga, Koh; Peterlin, Matija B
Naslov     Binding of Tat to TAR and recruitment of positive transcription elongation factor b occur independently in bovine immunodeficiency virus
Tip     članek
Vir     J Virol
Vol. in št.     Letnik 74, št. 13
Leto izdaje     2000
Obseg     str. 6039-44
ISSN     0022-538X
Jezik     eng
Abstrakt     Transcriptional transactivators (Tat) from many lentiviruses interact with their cognate transactivation response RNA structures (TAR) to increase rates of elongation rather than initiation of transcription. For several of them, the complex of Tat and a species-specific cyclin T1 must be formed before the binding to TAR can occur with high affinity and specificity. In sharp contrast, Tat from the bovine immunodeficiency virus (BIV) binds to its TAR without the help of the cyclin T1. This binding depends on the upper stem and 5' bulge, but not the central loop in TAR. Moreover, cyclins T1 from different species can mediate effects of this Tat in cells. Unlike the situation with other lentiviruses, Tat transactivation can be rescued simply by linking a heterologous promoter to TA in permissive cells. Thus, lentiviruses have evolved different strategies to recruit Tat and the positive transcription elongation factor b to their promoters, and interactions between Tat and TAR are independent from those between Tat and the cyclin T1 in BIV.
Deskriptorji     GENE PRODUCTS, TAT
IMMUNODEFICIENCY VIRUS, BOVINE
PROTEIN-SERINE-THREONINE KINASES
RNA, VIRAL
TRANS-ACTIVATION (GENETICS)
SPECIES SPECIFICITY
CATTLE
COS CELLS
CULTURE MEDIA, SERUM-FREE
CYCLINS
HELA CELLS
PROTEIN BINDING
DOGS