Avtor/Urednik		Sepčić, Kristina; Mancini, Ines; Vidic, Iztok; Jovan, Uroš; Frassanito, Rita; Pietra, Francesco; Maček, Peter; Turk, Tom
Naslov		Antibacterial and anticholinesterase activities of aplysamine-4, a bromotyrosine-derived metabolite of a red sea marine sponge
Tip		članek
Vir		J Nat Toxins
Vol. in št.		Letnik 10, št. 3
Leto izdaje		2001
Obseg		str. 181-91
Jezik		eng
Abstrakt		Aplysamine-4, a metabolite of likely bromotyrosine biogenesis, was isolated from an unidentified verongid sponge from the Red Sea. The compound was identified by heteronuclear magnetic resonance experiments, and by electrospray ionization tandem mass spectrometry. The compound exhibited moderate inhibitory activity on several Gram positive and Gram negative bacteria, and was also found to be a non-competitive reversible inhibitor of acetyicholinesterase. At pH 7.4, a K value of 16 and 2 uM was determined with electric eel and insect recombinant acetylcholinesterase, respectively. A deprotonated form of aplysamine-4 was obtained by alkaline treatment of the natural compound and it was shown to be less active than the protonated form.
Deskriptorji		TYROSINE BROMIDES ACETYLCHOLINESTERASE PORIFERA NUCLEAR MAGNETIC RESONANCE GRAM-POSITIVE BACTERIA GRAM-NEGATIVE BACTERIA COLONY COUNT, MICROBIAL INDIAN OCEAN