Avtor/Urednik		Turk, M; Gunde-Cimerman, N; Plemenitaš, A
Naslov		Activation of HOG pathway in halophilic black yeast Hortaea werneckii
Tip		članek
Vir		NATO Sci Ser, Gen Sub-Ser A Life Sci
Vol. in št.		Letnik 318
Leto izdaje		2001
Obseg		str. 162-7
Jezik		eng
Abstrakt		Many hypersaline environments are the preferred habitats of some microorganisms. Among them is also Hortaea werneckii, which belongs to the halophilic black yeasts, extremophilic eukaryotes, only recently isolated from hypersaline waters of salterns in the Mediterranean area in Slovenia and Spain as their natural environment. Since salterns are an extreme environment, a broad variety of biochemical adaptations have evolved to allow the organisms to cope with salt stress. Changes in membrane composition and membrane properties were found in halophilic H. werneckii. The high-osmolarity signalling pathway (I-IOG pathway) plays an important role in adapting to hyperosmotic stress in S. cerevisiae. Exposure of S. cerevisiae to increased extracellular osmolarity activates the Hogl mitogen activated protein kinase (MAPK). Activation of Hogl MAPK results in an induction of a set of osmoadaptive responses, which allow cells to survive in high-osmolarity environments. While many components of HOG pathway have been already identified in S. cerevisiae, little is known about this pathway in halophilic eukaryotic microorganisms. In this study the in vitro kinase assay measured by the activity of Hoglp revealed the difference in the activation of HOG pathway in halophilic H. werneckii exposed to different NaCI concentrations, when compared to mesophilic S. cerevisiae.
Deskriptorji		YEASTS SEAWATER OSMOLAR CONCENTRATION SIGNAL TRANSDUCTION PROTEIN KINASES BLOTTING, WESTERN SODIUM CHLORIDE