Avtor/Urednik		Zorc, Metka; Zorc-Pleskovič, Ruda; Petrovič, Danijel
Naslov		Histological diagnosis: apoptosis and proliferation markers as prognostic factors for the decision of surgical therapy in end-stage heart failure
Tip		članek
Vir		In: Štiblar-Martinčič D, Petrovič D, editors. Cardiovascular diseases. Proceedings of the 32nd memorial meeting devoted to professor Janez Plečnik, International symposium in memory of professor Rene Favaloro; 2001 Dec 6-8; Ljubljana. Ljubljana: Medical faculty,
Leto izdaje		2001
Obseg		str. 79-83
Jezik		eng
Abstrakt		The aim of this study was to evaluate the role of apoptosis, proliferation markers, volume density of interstitium, and myofibril volume fraction for the prognosis in patients with endstage dilated cardiomyopathy (DCM). Endomyocardial biopsy was performed during openheart surgery in 56 patients with end-stage DCM. Patients were divided into two groups, one group with shorter survival (24 ž 9 months, mean +- standard deviation) and another group with survival of more than 7 years after operation. The TUNEL method was used for the detection of apoptosis, and immunohistochemical methods were used for the evaluation of inhibitor of apoptosis (bcl-2), and proliferation markers (PCNA, Ki-67). The increased percentage of apoptotic myocytes, decreased expression of bcl-2, and decreased expression of PCNA and Ki-67 antigen was found in the group with early mortality compared to that with longer survival. Myofibril volume fraction was lower and volume density of interstitium was higher in the group with early mortality compared to that with longer survival. We may conclude that apoptosis, bcl-2 expression and proliferation activity of myocytes, myofibril volume fraction, and volume density of interstitium might be useful in predicting the prognosis (progressive versus non-progressive form) of patients with heart failure due to DCM.
Deskriptorji		HEART FAILURE, CONGESTIVE ENDOCARDIUM BIOPSY MYOFIBRILS CARDIAC OUTPUT APOPTOSIS