Avtor/Urednik		Kreft, M; Kreft, S
Naslov		Histolocalisation and computer aided reconstruction of polyphenol distribution in seeds
Tip		članek
Vir		In: Kreft I, Škrabanja V, editors. European scientific symposium Molecular and genetic interactions involving phytochemicals. COST 916 action: bioactive plant cell wall components in nutrition and health; 2000 Sep 17-20; Gozd Martuljek. Ljubljana: Biotehniška fakulteta,
Leto izdaje		2001
Obseg		str. 211-4
Jezik		eng
Abstrakt		Computer aided three-dimensional reconstruction was used to analyze the two dimensional images obtained by progressively grinding of buckwheat seeds. The seeds were embedded into polyester resin and 100 micro m thick slices were progressively removed. Each newly exposed plane was treated with the FeCl3 to produce a dark blue color with polyphenols and photographed. The photographs were imported into Silicon graphics computer and three-dimensional picture of the distribution of polyphenols was reconstructed. On the three dimensional reconstruction of the seed the distribution of polyphenols can be observed. The method is feasible to be applied to study the distribution of polyphenols as well in other seeds or plant parts with a complex structure.
Deskriptorji		SEEDS CEREALS COTYLEDON IMAGE PROCESSING, COMPUTER-ASSISTED PHENOLS