Avtor/Urednik		Geginat, Gernot; Schenk, Simone; Škoberne, Mojca; Goebel, Werner; Hof, Herbert
Naslov		A novel approach of direct ex vivo epitope mapping identifies dominant and subdominant CD4 and CD8 T cell epitopes from Listeria monocytogenes
Tip		članek
Vir		J Immunol
Vol. in št.		Letnik 166, št. 3
Leto izdaje		2001
Obseg		str. 1877-84
Jezik		eng
Abstrakt		We used a novel approach for the direct ex vivo identification and characterization of T cell epitopes based on the screening of peptide spot libraries with freshly isolated splenocytes in a sensitive enzyme-linked immunospot (ELISPOT) assay. This technique was applied for the analysis of splenocytes from Listeria monocytogenes-infected BALB/c and C57BL/6 mice. The screening of peptide spot libraries covering the whole listeriolysin O and p60 of L. monocytogenes confirmed all known CD4 and CD8 T cell epitopes of these proteins and additionally revealed six new H-2(d) and six new H-2(b)-restricted T cell epitopes. New epitopes were categorized into CD4 and CD8 T cell epitopes by ex vivo ELISPOT analysis with separated T cell populations.The quantitative analysis of cells reactive with these CD4 and CD8 T cell epitopes revealed the existence of dominant and subdominant CD4 and CD8 T cell populations during L. monocytogenes infection. As a consequence of these data we suggest that ELISPOT-based screening of peptide spot libraries could be a general approach for the rapid identification and characterization of pathogen-specific T cell populations during various infectious diseases.
Deskriptorji		LISTERIA MONOCYTOGENES EPITOPE MAPPING EPITOPES, T-LYMPHOCYTE CD4-POSITIVE T-LYMPHOCYTES CD8-POSITIVE T-LYMPHOCYTES MICE, INBRED BALB C MICE, INBRED C57BL T-LYMPHOCYTE SUBSETS PEPTIDE LIBRARY LYMPHOCYTE TRANSFORMATION IMMUNOPHENOTYPING IMMUNOLOGIC MEMORY HISTOCOMPATIBILITY ANTIGENS CLASS I HISTOCOMPATIBILITY ANTIGENS CLASS II HEAT-SHOCK PROTEINS CELL LINE ENZYME-LINKED IMMUNOSORBENT ASSAY BACTERIAL PROTEINS