Avtor/Urednik		Gašperšič, Rok
Naslov		Vpliv tumorje nekrotizirajočega dejavnika alfa (TNFalfa) na kostni metabolizem in eksperimentalni parodontitis pri poskusnih živalih
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2001
Obseg		str. 70
Jezik		slo
Abstrakt		Periodontal disease is an inflammatory disease of periodontal tissues, leading to degradation of periodontal tissues and alveolar bone loss. Inflammatory reaction is characterised by release of some enrymes, inflammatory mediators and cytokines from structure and inflammatory cells. Proinflammatory cytokine tumor necrosis factor alpha (TNFa) was found in cervicular fluid and periodontal tissues of patients with periodontal disease and several in vitro experiments have demonstrated it's potential osteoresorptive action. The aim of the first experiment was to demonstrate the osteoresorptive capacity of TNFa in vivo. Our hypothesis was that TNFa activates catabolic bone metabolism reactions in mice. Our second hypothesis was, that chronic infusion of TNFa accelerates the inflammatory reaction and promotes the progression of experimental periodontitis in rats. In our first experiment, 20 male C57-Black mice were used. Using microosmotic pumps ALZET, mouse recombinant TNFa was delivered subcutaneously (3pg per day) (n=5) or in tissues above the parietal bone (lpg per day) (n=5). Animals infused similarly with PBS served as a control group. Serum ionisied calcium and anorganic phosphates were evaluated colorimetricaly. Parietal bone osteoclasts were marked histochemicaly, and surface density of these cells were evaluated morphometricaly. In the second experiment, male Wistar rats were used. Inflammation of periodontal tissues was initiated by placing silk ligatures around the cervices of the right second maxillary molars. After two days, the microosmotic pumps ALZET were implanted subsutaneously for two weeks, containing human recombinant TNFalpha (3 micro g/day (n=5) of PBS. Serum ionised calcium and anorganic phosphate levels were evaluated colorimetrically, as in the first experiment. (Abstract truncated at 2000 characters).
Deskriptorji		PERIODONTITIS TUMOR NECROSIS FACTOR PARIETAL BONE PERIODONTAL ATTACHMENT LOSS OSTEOCLASTS MICE, INBRED C57BL RATS, WISTAR DISEASE MODELS, ANIMAL HISTOCYTOCHEMISTRY COLORIMETRY CALCIUM PHOSPHORUS ACID PHOSPHATASE