Avtor/Urednik		Remškar, Mojca
Naslov		Vpliv celičnega imunskega sistema na zadebelitev intime po poškodbi žilne stene
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2002
Obseg		str. 59
Jezik		slo
Abstrakt		Vessel wall injury induces remodeling and proliferation of smooth muscle cells and extracellular matrix, which results in vessel wall constriction and intimal thickening. This leads to vessel lumen narrowing which significantly limits the effectivness of therapeutic procedures used in modern cardiology. Immune system has been demonstrated to play an important role in the development of intimal thickening. We performed a study in which we investigated the effect of T lymphocytes and CD40 receptor (CD40) / CD40 ligand (CD40L) system on intimal thickening following acute mechanical vessel wall injury induced by placement of a flexible unobstructive sillicone collar around carotid artery: two control groups of B6/129 mice, which were injected with saline or IgG isotype antibody; splenectomized B6/129 mice, with a decreased T lymphocyte concentration in peripheral blood; B6/129 mice injected with monoclonal CD4 and CD8 antibodies which almost completely depleted T lymphocytes in peripheral blood; genetically modified mice without CD40L on their cells; and mice without CD40L on their cells injected with splenocytes of B6/129 mice that expressed CD40L. T lymphocyte concentration in peripheral blood was followed by flow cytometry. 21 days after injury mice were sacrifized and total intimal and medial area was measured. By immunohistochemical methodes we determined the expression of adhesive molecule VCAM-1 and collagen in vessel wall. In mice injected with monoclonal CD4+ and CDB+ antibodies only 2.3?3.1% of T lymphocytes were present in peripheral blood, 9.9?4.0% of T lymphocytes were present in peripheral blood of splenectomized mice and 26.4?6.1% of T lymphocytes in control groups of mice (p=0.04). In control group of mice 11?4% of T lymphocytes expressed CD40L on their surface before injury and 38?17% 24 hours after injury (p=0.04). (Abstact truncated at 2000 characters).
Deskriptorji		CAROTID ARTERIES TUNICA INTIMA ANTIGENS, CD40 RECEPTORS, ANTIGEN, T-CELL IGG MICE SPLENECTOMY FLOW CYTOMETRY IMMUNOHISTOCHEMISTRY VASCULAR CELL ADHESION MOLECULE-1 LYMPHOCYTE DEPLETION ANTIBODIES, MONOCLONAL CD4-POSITIVE T-LYMPHOCYTES CD8-POSITIVE T-LYMPHOCYTES