Avtor/Urednik		Vodopivec, M; Podgornik, A; Berovič, M; Štrancar, A
Naslov		Application of convective interaction media (CIM) disk monolithic columns for fast separation and monitoring of organic acids
Tip		članek
Vir		J Chromatogr Sci
Vol. in št.		Letnik 38, št. 11
Leto izdaje		2000
Obseg		str. 489-95
Jezik		eng
Abstrakt		The separation of organic acids on the anion-exchange monolithic support, commercially available as Convective Interaction Media (CIM), is presented in this study. It is demonstrated that citric, isocitric, pyruvic, fumaric, malic, and alpha-ketoglutaric acid can be successfully separated using a CIM monolithic column of suitable user-adjustable length. The effect of the mobile phase composition on the separation is investigated. CIM monolithic columns of adjustable length from 3 to 18 mm are compared regarding the resolution and the back pressure. It is shown that the CIM monolithic column of 12 mm in length enables a good separation of all six organic acids within 3 min and exhibits a linear dependence of back pressure versus flow rate. The resolution and the dynamic binding capacity are found to be flow-unaffected. A filtrated sample of bioprocess supernatant is analyzed without previous pretreatment, which indicates the possibility of online monitoring of small molecules during the bioprocess using CIM monolithic columns.
Deskriptorji		CHROMATOGRAPHY, ION EXCHANGE CITRIC ACID ISOCITRATES PYRUVIC ACID FUMARATES MALATES KETOGLUTARIC ACIDS