Avtor/Urednik		Podgornik, H; Stegu, M; Podgornik, A; Perdih, A
Naslov		Isolation and characterization of Mn(III) tartrate from Phanerochaete chrysosporium culture broth
Tip		članek
Vir		Fems Microbiol Lett
Vol. in št.		Letnik 201, št. 2
Leto izdaje		2001
Obseg		str. 265-9
Jezik		eng
Abstrakt		High initial Mn(II) concentration results in accumulation of a Mn(III) tartrate complex in the growth medium of Phanerochaete chrysosporium. Since Mn(III) is the major oxidant in ligninolysis by manganese peroxidase, the role of accumulated complex should not be neglected when degradation experiments by a crude culture filtrate are performed. To study the Mn(III) complex oxidative potential it was isolated by absorption to polyamide followed by desorption with an alkaline methanol solution. High performance liquid chromatography analysis and atomic absorption spectroscopy confirmed that the isolate was Mn(III) tartrate. Oxidation of 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonate) was used for testing the temperature and pH stability of the isolate that also intensively oxidized 2,6-dimethoxyphenol. In comparison with the non-isolated complex in the culture filtrate, the isolate showed increased temperature and pH stability. The oxidative potential of the isolated Mn(III) tartrate was additionally tested by decolorization of the synthetic dye Indigo carmine.
Deskriptorji		TARTRATES BASIDIOMYCETES CULTURE MEDIA, CONDITIONED TEMPERATURE ULTRAFILTRATION SULFONIC ACIDS CHROMATOGRAPHY, HIGH PRESSURE LIQUID HYDROGEN-ION CONCENTRATION PEROXIDASES OXIDATION-REDUCTION MOLECULAR WEIGHT HYDROGEN-ION CONCENTRATION