Avtor/Urednik		Pflegerl, K; Podgornik, A; Berger, E; Jungbauer, A
Naslov		Direct synthesis of peptides on convective interaction media monolithic columns for affinity chromatography
Tip		članek
Vir		J Com Chem
Vol. in št.		Letnik 4, št. 1
Leto izdaje		2002
Obseg		str. 33-7
Jezik		eng
Abstrakt		Solid-phase peptide synthesis was performed on glycidyle methacrylate-co-ethylene dimethacrylate monoliths using Fmoc chemistry. The native epoxy groups were amino-functionalized by reaction with ethylenediamine or ammonia ions. A peptide directed against human blood coagulation factor VIII was synthesized as a model peptide. Amino acid analysis revealed the correct amino acid ratio as present in the sequence. The ligand density of 5 micromol/mL was equal to that achieved with conventional peptide immobilization via epoxy groups. These supports were directly used as peptide affinity chromatography matrixes. The functionality of the CIM monolithic supports was proven by affinity chromatography of factor VIII. The ammonia-functionalized support performed with low hydrophobicity and did not show unspecific adsorption of proteins.
Deskriptorji		PEPTIDES CHROMATOGRAPHY, AFFINITY METHACRYLATES EPOXY COMPOUNDS ETHYLENEDIAMINES FACTOR VIII AMINO ACID SEQUENCE LIGANDS